Journal: International Journal of Molecular Sciences
Article Title: Toxicity of Jegosaponins A and B from Styrax japonica Siebold et al. Zuccarini in Prostate Cancer Cells and Zebrafish Embryos Resulting from Increased Membrane Permeability
doi: 10.3390/ijms22126354
Figure Lengend Snippet: Jegosaponin A and B increase cell membrane permeability. ( a ) Haemolysis of RBCs by jegosaponin A and B. n = 3, error bars indicate SD. ( b ) Increase in cell membrane permeability of PC-3 cells treated with jegosaponin A and B. Purple indicates membrane impermeable DRAQ7-stained nucleus. ( c ) Quantitative analysis of ( b ). n = 3, error bars indicate SD. * p < 0.05 and ** p < 0.01 vs. control (0 μM jegosaponin A); # p < 0.05 and ## p < 0.01 vs. control (0 μM jegosaponin B). ( d ) Increase of cell membrane permeability of 48 hpf zebrafish by jegosaponin A and B. Purple indicates membrane impermeable DRAQ7-stained nucleus.
Article Snippet: PC-3 cells were seeded (4 × 10 3 cells/well) in ibidi 8-well μ-Slide (Ibidi, Munich, Germany) in DMEM supplemented with 10% FBS and cultured for 24 h. Then, DRAQ7 (DRAQ7 DROP & GO; Biostatus, Shepshed, UK) and the tested compounds were added to the cells.
Techniques: Membrane, Permeability, Staining, Control